Human Ephrin-B2 is encoded by the EFNB2 gene, a EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors (Bonaldo, et al., 1994). EFNB2 is a member of the family of transmembrane-anchored ligands of the ephrin receptor tyrosine kinase family, the largest subgroup of receptor tyrosine kinases known (Poliakov, et al., 2004; Pasquale, 2004) and are type I membrane glycoproteins. Ephrin receptors and their ligands are known to participate in cell-cell interactions, including those of vascular endothelial cells, and are modulators of cell migration in remodeling events, especially in the nervous system. EFNB2 expression is seen in most human tissues to varying degrees. Hendra virus (HeV) and Nipah virus (NiV) belong to the genus Henipavirus of the family Paramyxoviridae and are unique in that they exhibit a broad species tropism and cause fatal disease in both animals and humans. They infect cells through the same pH-independent membrane fusion process mediated by their fusion and attachment glycoproteins. EFNB2 has been shown to serve as a functional receptor for both Hendra virus (HeV) and Nipah virus (NiV). Non-permissive cells transfected with a vector encoding EFNB2 became permissive for HeV- and NiV-mediated cell fusion as well as infection by live virus. Additionally, recombinant EFNB2 could potently block fusion and infection and bind recombinant HeV and NiV attachment glycoproteins with high affinity (Bonaparte, et al., 2005).
Recombinant protein corresponding to aa18-226 from human Ephrin B2 (exodomain), fused to 6XHis-Tag at C-terminal, expressed in HEK293 cells.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293
Grade：Highly Purified
Purity：≥95%. Purified by culture supernatant
Form：Supplied as a liquid in DPBS, pH 7.4, 0.09% sodium azide.
