Chlamydia trachomatis is propagated to maximum density in McCoy cells under Category 2 safety conditions. Infected cells are carefully washed and disrupted into buffered saline (PBS), subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris. Chlamydia antigen production is scalable from 10ml to several liters and is optimized by the multiplicity of infection and by harvesting time.
Source:Chlamydia trachomatis LGV2, from strain 434, from McCoy cells
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Heat treatment and 0.05% Triton x-100. Tested inactive blind passages on permissive cells showing no signs of inclusion bodies.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Source Antigen：Chlamydia trachomatis LGV2, from strain 434, from McCoy cells
Grade：Lysate
Purity：Lysate
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Triton X-100.
