Adenoviruses are medium-sized (80–100nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).
Wild-type adenoviruses, including human adenovirus type 5, are associated with a number of mild disorders, such as lower respiratory tract infections in the elderly or children. Of the 57 human adenovirus serotypes, 5 (Ad5) is one of the best studied, providing a better understanding of virus biology, host cellular processes and virus-cell interactions during infection. Ad5 is also widely used for transgene delivery in preclinical and clinical gene therapy studies; replication-defective vectors have been described expressing Ebola glycoproteins and malaria antigens for vaccine trials.
Concentrated and purified virus particles from a double CsCl gradient purification with DNase treatment and dialysis.
Source:Human adenovirus type 5 particles (wild-type), expressed in HEK293 cells
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Source Antigen：HEK293 cells
Grade：Highly Purified
Purity：≥99%
Form：Supplied as a liquid in PBS, pH 7.8, 50mM HEPES, 10% glycerol.
