Adenoviruses are medium-sized (80–100nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999). Adenovirus type 5 is one of the most extensively studied and characterized adenoviruses and is the type used most frequently in generating recombinant adenoviruses for gene therapy. These vectors generally contain deletions of the E1 and E3 genes, which allows for insertion and packaging of up to 7.5kb of foreign DNA, for gene delivery. This recombinant human adenovirus type 5 expresses luciferase protein under the control of a CMV promoter. Luciferases are naturally occurring protein enzymes that catalyze emission of light from a substrate (luminescence). Light is produced as a by-product of a reaction in which a luciferase enzyme converts a substrate to a product. Luciferase imaging with this vector can therefore be used to monitor virus trafficking, optimise virus transduction efficiency and determine viral infection conditions in specific cell types.
Concentrated and purified virus particles from a double CsCl gradient purification with DNase treatment and dialysis.
Source:Ad-luc, Luciferase, with an E1 and E3 deletion, and insertion of CMV-driven Luciferase gene in E1 region, expressed in 293AD cells.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Source Antigen：293AD cells
Grade：Highly Purified
Purity：≥99%
Form：Supplied as a liquid in PBS, pH 7.8, 50mM HEPES, 10% glycerol.
