Adenoviruses are medium-sized (80-100nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).
Human adenovirus type 3 has a worldwide distribution and is a major cause of febrile respiratory illness, predominantly in children and adolescents, that may or may not involve conjunctivitis.
Source:Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified on CsCl gradient.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Particle Count:2.53x10e12 particles/ml (BCA)
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Source Antigen：HEK293 cells
Grade：Highly Purified
Purity：≥99%
Form：Supplied as a liquid in PBS, pH 7.8, 50mM HEPES, 7.5% sucrose.
