Vicia villosa Lectin (VVL/VVA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.
Source:Hairy vetch
Activity:≥250ug/ml is usally required to agglutinate type A1 cells. ≤1ug/ml will agglutinate neuraminidase cells.
Sugar Specificity:N-Acetylgalactosamine
Inhibitory Carbohydrate:N-Acetylgalactosamine
Divalent Ions:Ca++, Mn++
Excitation:495nm
Emission:515nm
Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Source Antigen：Hairy vetch
Grade：Affinity Purified
Purity：Affinity purified
Form：Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 0.05mM CaCl, 0.05mm MnCl, 1% BSA, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
