85-6024-79　［取扱停止］Sambucus nigra (SNA/EBL II) 5mg　518749

特徴

• Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues. The blood group A-active trisaccharide containing L-fucosyl group linked alpha1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc alpha1-3Gal), which suggests steric hindrance to binding by the alpha-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity. EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.
• Source:Elderberry
• Blood Specificity:B > A > O
• Sugar Specificity:N-Acetylgalactosamine > Galactose
• Inhibitory Carbohydrate:Lactose > beta-Galactose
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.