Horseradish peroxidase (HRP) is a 40kD protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Maackia amurensis Lectin (MAA/MAL II) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.
Source:Amur maackia
Carbohydrate Specificity:a2-3 linked sialic acids
Inhibitory Carbohydrate:a2-6 sialullactose
Mitogenic Activity:Yes
Divalent Ions:None Required
Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:2mg
Source Antigen:Amur maackia
Form:Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 005mM CaCl, 0.05mM MnCl, 1% BSA. Labeled with HJrseradish peroxidase (HRP).