Alkaline phosphatase (AP) is conjugated to Galanthus nivalis Lectin (GNL/GNA) to show the binding of GNL/GNA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.
Source:Snowdrop
Blood Specificity:Rabbit Agglutinin
Sugar Specificity:alphaMannose
Inhibitory Carbohydrate:alpha-Methylmannoside
Divalent Ions:None Required
Storage and Stability:Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size:1mg
Source Antigen:Snowdrop
Grade:Highly Purified
Purity:Highly Purified
Form:Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Alkaline Phophatase (AP).