Euonymus europaeus Lectin (EEL/EEA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.
Source:Spindle tree
Blood Specificity:O (–SA), B
Sugar Specificity:Galalpha; 3Gal
Inhibitory Carbohydrate:Lactose
Fluorescence:Green
Divalent Ions:Ca++, Zn++
Excitation:495nm
Emission:515nm
Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:2mg
Source Antigen:Spindle tree
Grade:Affinity purified
Purity:Affinity purified
Form:Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).