Alkaline phosphatase (AP) is conjugated to Euonymus europaeus Lectin (EEL/EEA) to show the binding of EEL/EEA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.
Source:Spindle tree
Blood Specificity:O (–SA), B
Sugar Specificity:Galalpha; 3Gal
Inhibitory Carbohydrate:Lactose
Divalent Ions:Ca++, Zn++
Storage and Stability:Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：1mg
Source Antigen：Spindle tree
Grade：Highly Purified
Purity：Highly Purified
Form：Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Alkaline Phophatase (AP).
