Allium sativum lectin (ASA) is purified first with fractionation and then with gel filtration. It is a dimer of two subunits. ASA binds to a number of alpha1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity.
Source:Garlic
Blood Specificity:Rabbit Agglutinin
Sugar Specificity:Mannose
Inhibitory Carbohydrate:alpha(1,3)-Linked Mannosyl Units
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
