This BioAssay™ kit uses a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of synaptophysin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:177.4pg/ml
Range:493.8-40,000pg/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Synaptophysin (SYP). No significant cross-reactivity or interference between Synaptophysin (SYP) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to synaptophysin has been pre-coated onto a microplate. A competitive inhibition reaction is launched betweenbiotin labeled synaptophysin and unlabeled synaptophysin (Standards or samples) with the pre-coated antibody specific to synaptophysin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of synaptophysin in the sample. After addition of thesubstrate solution, the intensity of color developed is reverse proportional to the concentration of synaptophysin in the sample.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
