This sandwich ELISA kit is used for in vitro quantitative measurement of S100A4 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity:30pg/ml
Range:78-5000pg/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of High Sensitive S100 Calcium Binding Protein A4 (S100A4). No significant cross-reactivity or interference between High Sensitive S100 Calcium Binding Protein A4 (S100A4) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:The microplate provided in this kit has been pre-coated with an antibody specific to S100A4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to S100A4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100A4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
