This BioAssay™ kit uses competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of neurogranin in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 96% homology of the sequence among different species, the kit can be used to detect human,mouse and rat samples.
Sensitivity:7pg/ml
Range:16-10,000pg/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Neurogranin (NRGN). No significant cross-reactivity or interference between Neurogranin (NRGN) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to neurogranin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled neurogranin and unlabeled neurogranin (Standards or samples) with the pre-coated antibody specific to neurogranin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of neurogranin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of neurogranin in the sample.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
