Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intended Use:The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NGF in ovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:5.5pg/ml
Range:15.6-1000pg/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF). No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:The microplate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGF, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
