Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intended Use:The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IL17 in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:26.6pg/ml
Range:62.5-1000pg/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17). No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to IL17 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IL17 and unlabeled IL17 (Standards or samples) with the pre-coated antibody specific to IL17. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IL17 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IL17 in the sample.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
