Sample Type:Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intended Use:The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HIST1H4A in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:0.057ng/ml
Range:0.156-10ng/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Histone Cluster 1, H4a (HIST1H4A). No significant cross-reactivity or interference between Histone Cluster 1, H4a (HIST1H4A) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:The microplate provided in this kit has been pre-coated with an antibody specific to HIST1H4A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HIST1H4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIST1H4A, biotin-conjugatedantibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically ata wavelength of 450nm ± 10nm. The concentration of HIST1H4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
