The Heparanase (HPA) BioAssay™ ELISA Kit (Human), High Sensitivity, is a sandwich enzyme immunoassay for in vitro quantitative measurement of F1+2 in human serum, plasma and other biological fluids.
Sensitivity:0.04pg/ml
Range:0.11-80pg/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of High Sensitive Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between High Sensitive Prothrombin Fragment 1+2 (F1+2) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:The microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F1+2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
