Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intended Use:The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of butyrylcholinesterase in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:0.87ng/ml
Range:2.47-200ng/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Butyrylcholinesterase (BCHE). No significant cross-reactivity or interference between Butyrylcholinesterase (BCHE) and analogues was observed.
Precision:Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to butyrylcholinesterase has been pre-coated onto a microplate. A competitive inhibition reaction is launchedbetween biotin labeled butyrylcholinesterase and unlabeled butyrylcholinesterase (Standards or samples) with the pre-coated antibody specific to butyrylcholinesterase. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of butyrylcholinesterase in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of butyrylcholinesterase in the sample.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
