The Atrial Natriuretic Peptide (ANP) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ANP in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Precaution:The Stop Solution (517024K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store **517024A, **517024B, **517024D, **517024E and **517024J at -20°C. After reconstitution of **517024J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards;2. Add 50ul standard or sample to each well
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
