InstantBlue™ is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action stains proteins in 15 minutes, while leaving a clear background eliminating the need to fix, wash or destain. Formulated for safe use and easy disposal, it’s ready to use straight out of the bottle and comes in convenient premixed one liter volumes. Perfect for use with the InstantBlue™ Stain Tray.
The unique patented mechanism for rapid Coomassie blue staining of protein gels begins in moments, and results are achieved within 15 minutes. Some premade and traditional homemade Coomassie R-250 protein stains can take three hours or more to fully stain gels.
InstantBlue™ Protein Stain Features:There’s no need to wash, fix, microwave or destain gels when using InstantBlue™. Homemade stains often require all these steps and most premade solutions require at least one. InstantBlue™ uses a simple and easy to use one step protocol.
Unparalleled sensitivity allows protein levels as low as 5ng per band to be detected (BSA) when staining overnight. This is thanks to InstantBlue™’s protein specific staining, which gives a clear background, improving the signal/noise ratio of the stain. Despite the staining power, gels can be left in InstantBlue™ for weeks and still come out with a crystal clear background, all without the need for destaining or protein stain remover.
The nontoxic formula is sink disposable and thanks to its methanol free composition will not shrink gels. Other stains require solvent disposal procedures and can require microwaving, which produces dangerous fumes. The effective staining and safe composition of InstantBlue™ means that there’s no need for microwaving, solvent disposal, or fume hoods.
InstantBlue™ will stain at least 40 mini gels per bottle, boasting economical staining with only 25ml per mini gel. As a result, this allows you to use less protein staining solution when compared to 50–100ml of stain per gel for homemade solutions and some premixed solutions.
Fully compatible with mass spectrometry, gels can be destained using standard procedures. The protein staining solution’s acetic acid free formulation means it will not methylate or acetylate protein during the protein staining protocol.
Key Benefits:
Ultra fast staining – Results in 15 minutes or less
One step procedure – No washing, fixing, microwaving or destaining
High sensitivity – 5ng bands detectable
Clear background – High signal/noise ratio
Flexible – No overstaining
Reliable – Stringent batch to batch consistency
Efficient – Only 25ml per gel
Safe composition – Nontoxic; no fume hood or solvent disposal required
Methanol free – No gel shrinkage or protein methylation
Acetic acid free – No protein acetylation
Mass spectrometry compatible – Destainable. No residual methylation or acetylation.
