This assay kit is used for quantitative detection of in serum, plasma, and tissue homogenates.
Sensitivity:0.39ng/ml
Range:1.56-100ng/ml
Sample Volume:50-100ul
Detection Wavelength:450nm
Specificity:This assay has high sensitivity and excellent specificity for detection of human ECP. No significant cross-reactivity or interference between human ECP and analogues was observed.
Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
Assay Principle:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ECP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ECP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
