85-6000-43　［取扱停止］CD40 Ligand, Recombinant, Human, aa113-261 (CD40LG) 500ug　516800

特徴

• CD40LG protein is expressed on all B lymphocytes during various stages of development, activated T cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B cell malignancies and on some early B cell acute lymphocytic leukemias. Human CD40LG has been found to be the receptor for a protein involved in an X-linked immunodeficiency syndrome. Based on its homology with other members of the TNF ligand protein superfamily, CD40LG is also referred to also as TNFSF5. The soluble form of CD40LG is generated by the intracellular proteolytic processing of the full length CD40LG. Recombinant Human CD40LG is a 17kD protein that represents the soluble segment of full length CD40LG and encompasses the receptor binding TNF-like domain.
• Source:Recombinant partial protein corresponding to aa113-261 of human CD40 Ligand, expressed in E. coli.
• Molecular Weight:~16.2kD
• Biological Activity:The ED50 as determined by the dose-dependent stimulation of IL-12 and IL-8 induction by PMB cells is less than 20ug/ml.
• Endotoxin:<1EU/ug (LAL).
• AA Sequence:MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.