Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-d to play a fundamental role in the initiation of cell proliferation.
Recombinant protein corresponding to Proliferating Cell Nuclear Antigen (PCNA), fused to His-Tag, expressed in insect cells.
Molecular Weight:~29.6kD
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ug
Source Antigen：Recombinant, Sf21 cells (insect)
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Hepes, pH 7.5, 0.4M sodium chloride, 0.2mM Dithiothreitol, 0.5mM PMSF.
