Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66kD and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome.
Recombinant protein corresponding to Centromere Protein B (CENPB), fused to His-Tag, expressed in insect cells.
Molecular Weight:~80kD
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ug
Source Antigen：Recombinant, Sf21 cells (insect)
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Hepes-Sodium Hydroxide, pH 7.5, 0.15M sodium chloride, 0.02% sodium dodecylsulphate, 0.1mM PMSF.
