Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to sex hormones, to be specific, androgens and estrogens. Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin. SHBG levels are decreased by androgens, anabolic steroids. polycystic ovary syndrome, hypothyroidism, diabetes, obesity, Cushing’s syndrome, and acromegaly. SHBG levels increase with estrogenic states (oral contraceptives), pregnancy, hyperthyroidism, cirrhosis, anorexia nervosa, and certain drugs. Long-term calorie restriction of more than 50 percent increases SHBG, while lowering free and total testosterone and estradiol. DHEA-S, which lacks affinity for SHBG, is not affected by calorie restriction. Polycystic Ovarian Syndrome is associated with insulin resistance and excess insulin lowers SHBG, which increases free testosterone levels. Recombinant human SHBG is expressed in mammalian cells and purified by using conventional chromatography.
Recombinant protein corresponding to aa30-402 of human Sex Hormone-Binding Globulin, expressed in AD 293 cells (Derivative of HEK 293). Swiss/UniProt Accession: P04278
Molecular Weight:~43-47kD
Biological Activity:≥10nMol/ml
Bioburden:≤10cfu/ml
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：AD 293 cells (Derivative of HEK 293)
Grade：Highly Purified
Purity：≥95%
Form：Supplied as a liquid in 20mM sodium phosphate, pH 7.4, 100mM sodium chloride, 1mM AEBSF, 0.09% sodium azide, 5% glycerol.
