Cholera is an acute, diarrheal illness caused by infection of the intestine with the bacterium Vibrio cholerae. An estimated 3-5 million cases and over 100,000 deaths occur each year around the world. The infection is often mild or without symptoms, but can sometimes be severe. Approximately one in 10 (5-10%) infected persons will have severe disease characterized by profuse watery diarrhea, vomiting, and leg cramps. In these people, rapid loss of body fluids leads to dehydration and shock. Without treatment, death can occur within hours.
Cholera toxin is an oligomeric complex made up of six protein subunits: a single copy of the A subunit and five copies of the B subunit. Subunit B binds to the cell surface via GM1 gangliosides on the surface of target cells. Once bound, the entire toxin complex is endocytosed by the cell and the cholera toxin A1 (CTA1) chain is released by the reduction of a disulfide bridge. Once inside the cell subunit A activates G protein which then activates adenylate cyclase, eventually leading to enhanced efflux of chloride ions from the intestinal cells, and rapid water loss via the intestine.
Cholera toxin subunit B (CTXB) has been used as a marker for neuronal cells due to its high affinity for the GM1 ganglioside cell surface receptor on these cells. CTXB has no toxic activity by itself, and can therefore be used in cell culture.
Source:Recombinant protein corresponding to Cholera toxin subunit B, Thr22-Asn124, from serotype O1 of Vibrio cholerae, fused to 6xHis-Tag at C-terminal, expressed in HEK293 cells.
Strain:Serotype 01
Molecular Weight:~13.5kD (calculated), and reduced protein migrates as a band of ~18-22kD.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a lyophilized powder in DPBS, pH 7.4. Reconstitute with 250ul sterile ddH2O.
