Nipah virus glycoprotein G has a globular head domain formed of a six-bladed beta sheet-propeller, connected via a flexible stalk domain to a transmembrane anchor. The G protein binds to the cellular receptors ephrin B2 and ephrin B3, mediating viral attachment. Following attachment Nipah Virus glycoprotein G undergoes a conformational change that leads to triggering of glycoprotein F which leads to membrane fusion (Biering et al, 2012).
Nipah virus (NiV) is an enveloped single stranded negative sense RNA virus that belongs to the Henipavirus genus, which is a new member of the Paramyxoviridae family. Nipah infection was first recognised in Malaysia 1998/1999, where a major NiV outbreak occurred in pigs and humans. A subsequent outbreak of NiV in Singapore also pointed to porcine as an intermediate host. However, outbreaks in India and Bangladesh did not. The natural host for NiV has now been identified as the fruit bat, of the Pteropus genus, with porcine acting as intermediate host in some cases. Reports suggest that transmission of Nipah virus to humans can occur through contact with NiV infected bats, food contaminated by bat’s excrement, infected porcine and other NiV infected humans.
Nipah, the disease caused by NiV infection is now endemic in South Asia and several outbreaks of NiV infection have been reported in India and Bangladesh. The symptoms of Nipah virus infection in humans can include rapidly developing fever, abdominal pain, nausea, vomiting, acute respiratory syndrome and severe encephalitis, which is fatal in a high percentage of cases (WHO).
In 2015, the World Health Organization highlighted NiV infection as an emerging disease requiring accelerated R&D to advance in vitro diagnostic development, vaccine design and therapeutics (WHO, 2015).
Source:Recombinant protein corresponding to aa71-602 of Nipah Virus glycoprotein G, fused to a glycine-serine linker and a mouse IgG2a Fc-Tag at C-terminal, expressed in HEK293 cells.
Strain:Malaysia 2008
Molecular Weight:~110kD in reducing SDS-PAGE analysis
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
