85-5999-53　［取扱停止］HIV-1 [HIV-1/Clade E (CM244)] GP120, Recombinant, aa36-511, His-Tag (Human Immunodeficiency Virus-1) 100ug　506310

特徴

• HIV-1 viruses may be further divided into groups, being M, N, O and P. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Within the M group of HIV-1 there are a number of genetically distinct sub-types (also known as clades). Different subtypes can also combine genetic material to form a hybrid virus or “circulating recombinant form (CRF)”. Subtype B is the most common in the Americas and Western Europe, whilst subtype C is the predominant form in Africa and India. Most research has been carried out into subtype B, although it accounts for only around 12% of infections worldwide.
• HIV GP120 protein (or gp120) is the name of the glycoprotein which forms the spikes sticking out of a HIV virus particle. HIV gp120 protein is essential for virus entry into cells as it plays a vital role in seeking out specific cell surface receptors for entry. Three gp120s, bound as heterodimers to a transmembrane glycoprotein, gp41, are thought to combine in a trimer to form the envelope spike, which is involved in virus-cell attachment. Approximately 50% of the mass of HIV gp120 protein is due to glycosylation, the high level of which may prevent gp120 from being recognized by the human immune response. gp120 binds to the human cell surface antigen CD4, which is primarily expressed by Helper T lymphocytes and monocytes/macrophages.
• GP120 from strain CM244 was used in the AIDSVAX vaccine, originally developed at Genentech.
• Recombinant protein corresponding to Thr36-Lys511 from HIV-1 [HIV-1/Clade E (CM244)] GP120, fused to C-terminal 6X His-Tag, expressed in HEK293 cells.
• Molecular Weight:~54.1kD, migrates at ~65-110kD in reducing SDS-PAGE due to glycosylation
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.