This Zaire subtype of the Ebola virus family is currently the most important in relation to outbreaks of disease in humans. This subtype has been responsible for the largest ever outbreak of EHF, which started in West Africa in 2014, and was finally declared over only in early 2016. The Kikwit-95 strain was isolated from an outbreak occurring in the city of Kikwit in Zaire in 1995. This outbreak has been especially well studied. There were 314 cases, with 244 fatalities, a mortality rate of Greater than 75%.
Ebola virus envelope glycoprotein is initially produced as a precursor known as pre-GP, which is cleaved by furin into two subunits, GP1 and GP2, which remain associated through a disulfide linkage between Cys53 of GP1 and Cys609 of GP2. This heterodimer assembles into a 450kD trimer at the surface of nascent virions. The virion-attached GP is critical in the EBOV life cycle, as it is solely responsible for attachment, fusion and entry of target cells. Moreover, GP is responsible for critical pathogenic differences among viral species.
The role of Ebola virus envelope glycoprotein in EBOV pathogenesis is unclear, but is examined in detail by Lee and Saphire (2009).
Source:Recombinant protein corresponding to Ebolavirus Envelope Glycoprotein (GP), Ile33-Asp637, fused to C-terminal 6x His-tag, expressed in HEK293 cells
This Ebola virus envelope glycoprotein (GP) contains both GP1 and GP2, in heterodimeric form.
Subtype:Zaire
Strain:Kikwit-95
Molecular Weight:~67kD
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a lyophilized powder in DPBS, pH 7.4. Reconstitute with 500ul sterile ddH2O.
