85-5999-05　Crispr Mutation Detection Kit, BioGenomics™ 100Rxn　506207

特徴

• The Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN).
• The Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.
• Applications:Detect the gene mutagenesis and SNP, for cleavage efficiency assay caused by ZFNs, TALENs, CRISPR/Cas9 or other gene editing tools.
• Kit Components:High-Fidelity DNA polymerase: 25rxn: 10ul, 100rxn: 40ul5X PCR Reaction Buffer: 25rxn: 150ul, 100rxn: 600ulT7 Endonuclease I: 25rxn: 25ul, 100rxn: 100ul10X T7 Endonuclease I Reaction buffer: 25rxn: 50ul, 100rxn: 500ulControl Template DNA: 25rxn: 30ul, 100rxn: 120ulControl Primer Mix: 25rxn: 30ul, 100rxn: 120ulProtease K: 25rxn: 25ul, 100rxn: 100uldNTP: 25rxn: 15ul, 100rxn: 60ul
• Storage and Stability:Store all components at -20°C upon receipt.