85-5999-03　Cas9 Nuclease, Recombinant, Streptococcus pyogenes 50ug (5×10ug)　506206

特徴

• Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With Cas9 nuclease, customers can screen highly efficient gRNA using in vitro DNA cleavage assays. It could be a powerful tool for gene editing.
• Cas9 Nuclease is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes without nuclear localization signal (NLS).
• Applications:Screening for highly efficient and specific targeting gRNAs by in vitro DNA cleavage using Cas9 Nuclease from S. pyogenes. Highly purified Cas9 antigen could be used for specific antibody production.
• Supplied With:Reaction Buffer, 10X Supplied as a liquid in 100mM Tris-HCl, 300mM sodium chloride, pH 7, 0.1mM EDTA, 1mM DTT, 50% glycerol.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.