85-5998-71　KMT5A, Recombinant, Human, aa195-352, His-Tag (N-lysine Methyltransferase KMT5A Isoform 1) 50ug　506189

特徴

• KMT5A, also known as N-lysine methyltransferase KMT5A isoform 1, is an enzyme which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1). It is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. It acts as a barrier to prevent cellular senescence through chromatin-mediated regulation of senescence-associated metabolic remodeling. It mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. The loss of KMT5A concurrently stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism.
• Description:Recombinant protein corresponding to aa195-352 of human KMT5A, fused to His-tag at C-terminus, expressed in Baculovirus.
• Molecular Weight:~18.9kD (165aa), ~18-28kD (SDS-PAGE under reducing conditions)
• Endotoxin:<1EU/1ug (LAL)
• AA Sequence:MKAELQSEER KRIDELIESG KEEGMKIDLI DGKGRGVIAT KQFSRGDFVV EYHGDLIEIT DAKKREALYA QDPSTGCYMY YFQYLSKTYC VDATRETNRL GRLINHSKCG NCQTKLHDID GVPHLILIAS RDIAAGEELL YDYGDRSKAS IEAHPWLKHH HHHHH
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.