85-5997-99　［取扱停止］Toxin B, Clostridium difficile, Strain VPI10463 50ug　506156

特徴

• Clostridium difficile (C.difficile) is a gram positive spore-forming anaerobic bacterium, which was first described in the mid-1930s. Recent studies have shown that C.difficile is predominantly associated with cases of infectious diarrhea in patients that have been treated with antibiotics (antibiotic-associated diarrhea AAD), or have disrupted commensal gastrointestinal flora. C.difficile infection can cause severe disease and death in a significant number of cases and is recognized as a leading cause of severe gastrointestinal disease and AAD in hospitalized patients (Voth, DE). The severity of the disease in each case is determined by the virulence of the C.difficile strain, the condition of the patient’s normal gut flora and the individual’s immune response to intestinal damage.
• Toxins A and B have been identified as major C. difficile virulence factors, which are encoded by the tcdA and tcdB genes respectively. Both toxin A and toxin B have proinflammatory and cytotoxic activity, which cause disruption to the intestinal epithelium leading to extensive damage and cell death in the large intestine (Carter, GP).
• Native Toxin B, strain VPI10463 (toxinotype 0), from Clostridium difficile
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Verocytotoxicity Assay Result:2pg/ml (lowest point at which rounded cells are observed)
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.