The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Source:Partial length protein corresponding to of PPHLN1, fused to GST-Tag at N-terminal, expressed in E. coli.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight:~40kD
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ug
Source Antigen：E. coli, Recombinant
Grade：Lysate
Purity：Lysate
Form：Supplied as a lyophilized powder in Tris-HCl, pH 6.8, 0.2M DTT, 4% SDS, 0.2% bromophenol blue, 20% sucrose. Reconstitute with 200ul sterile dH2O.
