Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF)-like repeats or thrombospondin (THBS) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates.
Source:Partial length protein corresponding to of POFUT2, fused to GST-Tag at N-terminal, expressed in E. coli.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight:~47kD
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:200ug
Source Antigen:E. coli, Recombinant
Grade:Lysate
Purity:Lysate
Form:Supplied as a lyophilized powder. Reconstitute with 200ul sterile dH2O.