DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.
Source:Partial length protein corresponding to of DUT, fused to GST-Tag at N-terminal, expressed in E. coli.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight:~42kD
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ug
Source Antigen：E. coli, Recombinant
Grade：Lysate
Purity：Lysate
Form：Supplied as a lyophilized powder in Tris-HCl, pH 6.8, 0.2M DTT, 4% SDS, 0.2% bromophenol blue, 20% sucrose. Reconstitute with 200ul sterile dH2O.
