Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig. Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.
Recombinant protein corresponding to Tissue Transglutaminase (tTG), fused to His tag, expressed in Sf-21 Insect Cells
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight:~78kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Source Antigen：Recombinant, Sf-21 Insect Cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Hepes, pH 8.0, 0.32M sodium chloride, 0.5mM phenylmethanesulfonyl fluoride (PMSF), 8mM EDTA, 20% glycerol, proprietary formula containing 0.1% preservative, Bromo-Nitro-Dioxane/Methylisothiazolone.
