IGF2, as known as insulin-like growth factor 2, is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. This protein is a fetal growth factor, influenced by placental lactogen and abundantly expressed by placental trophoblasts.
Recombinant human IGF-2 is a single, non-glycosylated, polypeptide chain containing 67aa, expressed in E coli.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Biological Activity:The ED50 as determined by the dose-dependent proliferation of MCF-7 cells was found to be 0.85ng/ml.
Specific Activity:1,176 x 10e6 IU/mg.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~97% (RP-HPLC, SDS-PAGE).
Form：Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
