Influenza virus is a single-stranded RNA virus, segmented, 70nm diameter and enveloped. Strains are described by geographic origin, strain number, year of isolation and hemagglutination (H) and neuraminidase (N) antigens.
Source:Embryonated chicken eggs. Strain: H1N1
Applications:Suitable for use as a positive control for antigen detection and in ELISA for antibody detection. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
HA Titer:20K HA Units/mL
Inactivation:Gamma radiation inactivation. Effectiveness determined when no detection of growth in two blind passages under the original culture conditions. Result: No live virus detected by CPE or HA assay
This reagent has been inactivated and should contain no infectious material. Generally accepted good laboratory practices appropriate to biological reagents should be employed when handling this product.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity：Eggs are infected by the amniotic route, incubated for 72 hours and refrigerated overnight. Amniotic fluids are harvested from live eggs and the embryos are washed in PBS. The suspension was concentrated usingcross-flow ultracentrifugation system with 30,000 molecular weight cut off. The concentrate is sonicated and clarified using low speed centrifugation. This preparation contains a high concentration of viral antigens as well as some egg proteins in PBS.
