85-5989-92　Total Cytotoxicity Test BioAssay™ Kit 125Tests　474046

特徴

• Sample Type:Cell culture
• Reagent Name:7-AAD, CFSE, SR-VAD-FMK
• Target:Cell-mediated cytotoxicity, poly caspases
• Excitation/Emission:7-AAD–546nm/647nm; CFSE–492nm/520nm; SR-FLICA–565nm/600nm
• Method of Analysis:Flow cytometry
• Intended Use:Total Cytotoxicity & Apoptosis Assay is a single-tube, tri-color assay for quantitative assessment of cell-mediated cytolytic activity due to apoptosis and necrosis. The assay employs a green fluorescent cellular stain, CFSE, to label target cells, the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity assay samples, and the orange-red SR-FLICA reagent, SR-VAD-FMK, to measure caspase activity in the target cell population. Analyze your results using a flow cytometer.
• Test Principle:Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Total Cytotoxicity BioAssay™ allows you to assess cytolytic activity in cell culture.
• The Total Cytotoxicity & Apoptosis Assay includes three fluorescent reagents: CFSE, 7-AAD, and SR-FLICA. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. SR-FLICA reagent is incubated with cells to identify those containing active caspases. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. Through flow cytometry, quantify four populations of cells: live, early apoptotic, late apoptotic, and necrotic.
• Kit Components:125 tests:SR-FLICA Reagent (SR-VAD-FMK), 1 vialCFSE target cell stain, 1 vial7-AAD vital stain, 1 vial10X Assay Buffer, 1x30mL250 tests:SR-FLICA Reagent (SR-VAD-FMK), 2 vialsCFSE target cell stain, 1 vial7-AAD vital stain, 2 vials10X Assay Buffer, 1x60mL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.