85-5989-91　SR-FLICA® Poly Caspase BioAssay™ Kit 100Tests　474045

特徴

• Sample Type:Cell culture
• Reagent Name:SR-VAD-FMK
• Target:Poly caspases
• Excitation/Emission:550-580nm/590-600nm
• Method of Analysis:Flow Cytometer, Fluorescence Plate Reader, Fluorescence Microscope
• Intended Use:Detect active caspase enzymes with the SR FLICA Poly Caspase BioAssay™ Kit. This in vitro assay employs the fluorescent inhibitor probe SR-VAD-FMK to label active caspase enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
• Test Principle:Caspases play important roles in apoptosis and inflammation. FLICA poly caspase BioAssay™ kit is used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues.
• The FLICA reagent SR-VAD-FMK enters each cell and irreversibly binds to activated caspases. Because the SR-VAD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound SR-VAD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the active caspase enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the BioAssay™ kit. Unfixed samples may also be analyzed with Hoechst 33342 to detect changes in nuclear morphology.
• Kit Components:25 tests:FLICA Poly Caspase Reagent (SR-VAD-FMK), 1 vial10X Apoptosis Wash Buffer, 1x15mLFixative, 1x6mLHoechst 33342, 1x1mL100 tests:FLICA Poly Caspase Reagent (SR-VAD-FMK), 4 vials10X Apoptosis Wash Buffer, 1x60mLFixative, 1x6mLHoechst 33342, 1x1mL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.