85-5989-89　SR-FLICA® Caspase 9 BioAssay™ Kit 100Tests　474044

特徴

• Intended Use:Detect caspase-9 activity with the SR-FLICA Caspase-9 BioAssay™ Kit. This in vitro assay employs the fluorescent inhibitor probe SR-LEHD-FMK to label active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
• Test Principle:Caspases play important roles in apoptosis and inflammation. FLICA BioAssay™ kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues.
• The FLICA reagent SR-LEHD-FMK enters each cell and irreversibly binds to activated caspase-9. Because the SR-LEHD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound SR-LEHD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the active caspase-9 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the BioAssay™ kit. Unfixed samples may also be analyzed with Hoechst 33342 to detect changes in nuclear morphology.
• Excitation/Emission:550-580nm (peak @570nm)/590-600nm (peak @590nmHoechst 33342: 364/480nm
• Kit Components:*474044A: FLICA Caspase-9 Reagent (SR-LEHD-FMK), 1x1vial or 1x4vials 474044B: Apoptosis Wash Buffer, 10X 1x15ml or 1x60ml474044C: Fixative, 1x6ml474044D: Hoechst 33342, 1x1ml
• Storage and Stability:Store *474044A powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.