Method of Analysis:Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
Intended Use:The MitoPT TMRM BioAssay™ detects mitochondrial membrane depolarization utilizing the fluorescent dye TMRM. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, TMRM reagent is dispersed through the cell cytosol and fluorescence levels drop dramatically. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
Test Principle:Loss of mitochondrial membrane potential is indicative of apoptosis. MitoPT TMRM BioAssay™ utilizes the potentiometric fluorescent dye TMRM to detect mitochondrial membrane permeability and membrane depolarization.
The TMRM dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The TMRM dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT TMRM becomes distributed throughout the cytosol.
Detection of the loss of orange-red fluorescence in TMRM stained cells is a reliable method for assessing apoptosis induction or oxidative stress-induced mitochondrial depolarization in experimental cell populations.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
