85-5989-71　MitoPT® JC-1 BioAssay™ Kit 100Tests　474033

特徴

• Sample Type:Cell culture
• Reagent Name:JC-1
• Target:Mitochondrial depolarization
• Excitation/Emission:488nm/590nm, 527nm
• Method of Analysis:Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
• Intended Use:The MitoPT JC-1 BioAssay™ detects mitochondrial membrane depolarization utilizing the fluorescent dye JC-1. When accumulated in a negatively charged polarized mitochondria, JC-1 fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, JC-1 reagent is dispersed through the cell and will fluoresce green. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
• Test Principle:Loss of mitochondrial membrane potential is indicative of apoptosis. MitoPT JC-1 BioAssay™ utilizes the potentiometric fluorescent dye JC-1 to detect mitochondrial membrane permeability and membrane depolarization.
• The JC-1 dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The JC-1 dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT JC-1 becomes distributed throughout the cytosol. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
• Kit Components:100 tests:MitoPT JC-1 Reagent, 100 Tests10X Assay Buffer, 1x60mLCCCP, 50mM, 1x125uL400 tests:MitoPT JC-1 Reagent, 400 Tests10X Assay Buffer, 2x125mLCCCP, 50mM, 600uL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.