85-5989-63　Magic Red™ Caspase 3 & 7 BioAssay™ Kit 25Tests　474029

特徴

• Sample Type:Cell culture, tissue
• Target:Caspase 3 and 7
• Excitation/Emission:590nm/628nm
• Method of Analysis:Fluorescence Microscope, Fluorescent Plate Reader, Flow Cytometer
• Intended Use:Detect caspase 3 and 7 activity in real time with the Magic Red Caspase 3/7 BioAssay™ Kit. The Magic Red reagent fluoresces upon cleavage by active caspase 3 and 7 enzymes. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or flow cytometry.
• Test Principle:Caspases play important roles in apoptosis and inflammation. Detection of DEVDase enzyme activity (caspase 3 activity) is a reliable method for assessing apoptotic activity in experimental cell populations. The presence of the active form of caspase 3 & 7 in apoptotic cells causes the hydrolysis of the two DEVD target sequences from the Magic Red cresyl violet fluorophore, converting it to the fluorescent form. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases. Magic Red excites at 590nm and emits at 628nm and can be analyzed using fluorescence microscopy, a fluorescent plate reader, or flow cytometry. Samples may also be analyzed with Acridine Orange dye or Hoechst stain to visualize lysosomal organelle structure or detect changes in nuclear morphology respectively.
• Kit Components:25 tests:MR-(DEVD)2 Reagent, 1 25-test vialHoechst 33342 Stain, 1x1mLAcridine Orange Stain, 1x1mL100 tests:MR-(DEVD)2 Reagent, 1 100-test vialHoechst 33342 Stain, 1x1mLAcridine Orange Stain, 1x1mL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.