85-5989-46　FAM-Phe-CMK Green FLISP™ BioAssay™ Kit 25Tests　474016

特徴

• Sample Type:Cell culture
• Reagent Name:FFCK
• Target:Chymotrypsin-like enzymes
• Excitation/Emission:488nm/520nm
• Method of Analysis:Flow cytometry, Fluorescence microscope, fluorescence plate reader
• Intended Use:Quantitate intracellular chymotrypsin-like serine protease activity in vitro with FLISP BioAssay™ kits. Chymotrypsin-targeting FLISP probes interact with active catalytic sites of chymotrypsin-like proteases, so cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence potential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
• Test Principle:Because of their supporting role in the apoptotic process, serine protease activity will be greater in apoptotic cell populations compared to healthy cells of the same type. Quantitate intracellular chymotrypsin-like serine protease activity in vitro with FLISP BioAssay™ kits. If there is an active chymotrypsin-like enzyme inside the cell, it will covalently bind with the FLISP inhibitor, in this case FAM-Phe-CMK (FFCK), and retain the green fluorescent signal inside the cell. Cells containing lower concentrations of chymotrypsin-like enzyme activity will retain a lower level of fluorescence compared to cells containing higher concentrations of this effector enzyme component.
• FLISP may be used in combination with FLICA to discriminate serine protease activity from caspase activity in the same cell. Analyze results using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
• Kit Components:25 tests:FAM-Phe-CMK, 1 vialHoechst Stain, 1x1mLPropidium Iodide, 1x1mL10X Cellular Wash Buffer, 1x15mLFixative, 1x6mL100 tests:FAM-Phe-CMK, 4 vialsHoechst Stain, 1x1mLPropidium Iodide, 1x1mL10X Cellular Wash Buffer, 1x60mLFixative, 1x6mL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.