85-5989-45　FAM-Leu-DAP Green FLISP™ BioAssay™ Kit 100Tests　474015

特徴

• Sample Type:Cell culture
• Reagent Name:FLDAP
• Target:Chymotrypsin-like enzymes
• Excitation/Emission:488nm/520nm
• Method of Analysis:Flow cytometry, Fluorescence microscope, fluorescence plate reader
• Intended Use:Quantitate intracellular chymotrypsin-like serine protease activity in vitro with FLISP BioAssay™ kits. Chymotrypsin-targeting FLISP probes interact with active catalytic sites of chymotrypsin-like proteases, so cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence potential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
• Test Principle:Because of their supporting role in the apoptotic process, serine protease activity will be greater in apoptotic cell populations compared to healthy cells of the same type. Quantitate intracellular chymotrypsin-like serine protease activity in vitro with FLISP BioAssay™ kits. If there is an active chymotrypsin-like enzyme inside the cell, it will covalently bind with the FLISP inhibitor, in this case FAM-Leu-DAP (FLDAP), and retain the green fluorescent signal inside the cell. Cells containing lower concentrations of chymotrypsin-like enzyme activity will retain a lower level of fluorescence compared to cells containing higher concentrations of this effector enzyme component. FLISP may be used in combination with FLICA to discriminate serine protease activity from caspase activity in the same cell. Analyze results using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
• Kit Components:25 tests:FAM-Leu-DAP, 1 vialHoechst Stain, 1x1mLPropidium Iodide, 1x1mL10X Cellular Wash Buffer, 1x15mLFixative, 1x6mL100 tests:FAM-Leu-DAP, 4 vialsHoechst Stain, 1x1mLPropidium Iodide, 1x1mL10X Cellular Wash Buffer, 1x60mLFixative, 1x6mL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.