85-5989-39　FAM-FLICA® Caspase 8 BioAssay™ Kit 100Tests　474012

特徴

• Sample Type:Cell culture
• Reagent Name:FAM-LETD-FMK
• Target:Caspase-8
• Excitation/Emission:488nm/530nm
• Method of Analysis:Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
• Intended Use:Detect caspase-8 activity with the FLICA Caspase-8 BioAssay™ Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-LETD-FMK to label active caspase-8 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
• Test Principle:Caspases play important roles in apoptosis and inflammation. This FLICA BioAssay™ kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-8 assay probe allows researchers to assess caspase-8 activation.
• The FLICA reagent FAM-LETD-FMK enters each cell and irreversibly binds to activated caspase-8. Because the FAM-LETD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound FAM-LETD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase-8 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the BioAssay™ kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively.
• Kit Components:25 tests:FLICA Caspase-8 Reagent (FAM-LETD-FMK), 1 vial10X Apoptosis Wash Buffer, 1x15mLFixative, 1x6mLPropidium Iodide, 1x1mLHoechst 33342, 1x1mL100 tests:FLICA Caspase-8 Reagent (FAM-LETD-FMK), 4 vials10X Apoptosis Wash Buffer, 1x60mLFixative, 1x6mLPropidium Iodide, 1x1mLHoechst 33342, 1x1mL
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.